Coat and Enterotoxin-related Proteins in Clostridium perfringens Spores
نویسندگان
چکیده
منابع مشابه
Spore coat protein and enterotoxin synthesis in Clostridium perfringens.
Polyacrylamide gel profiles of Clostridium perfringens spore coat protein revealed four and occasionally five components. Pulse-chase experiments indicated that synthesis of coat protein polypeptide and enterotoxin was an early sporulation event. However, maximum synthesis occurred coincident with the onset of heat resistance.
متن کاملClostridium perfringens Sporulation and Enterotoxin Synthesis
5 Running title: Role of SigF and SigG in sporulation and CPE synthesis 6 * To Whom Correspondence Should be Addressed: 7 Bruce A. McClane 8 Department of Microbiology and Molecular Genetics 9 447 Bridgeside Point II Building 10 450 Technology Drive 11 University of Pittsburgh School of Medicine 12 Pittsburgh, PA 15219 13 Phone: 412-648-9022 14 Fax: 412-624-1401 15 Email: [email protected] 16 Copy...
متن کاملClostridium perfringens enterotoxin: a brief review.
Ussing chambers were mounted with stripped rabbit ileal mucosa and either crude or pure toxin A added to the mucosal side of the chamber to final concentrations of 800 and 30pg/ml respectively; increases in short-circuit currents of the order of 25pA were observed in both preparations over a period of 90min, at which point the tissue was reponsive to theophylline but not glucose. Histology show...
متن کاملAffinity chromatography purification of Clostridium perfringens enterotoxin.
Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Cell extracts containing enterotoxin or partially purified toxin preparations were applied to the column and nonspecifically-bound protein was eluted. NaOH was used to elute specifically bound toxin. The purity of enterotoxin p...
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Clostridium perfringens is one of the five major food borne pathogens in the western world (expressed in cases per year). Symptoms are caused by an enterotoxin, for which 6% of type A strains carry the structural gene. This enterotoxin is released when ingested cells sporulate in the small intestine. Research on C. perfringens has been limited to a couple of strains that sporulate well in Dunca...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1989
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-135-11-3109